What is Microscopy?

Electron microscope, referred to as Electron Microscope, English name Electron Microscope (EM for short), has become an indispensable important tool in modern science and technology after more than 50 years of development. The electron microscope consists of a lens barrel, a vacuum device and a power cabinet.

Electron microscope

Electron microscope, abbreviated as Electron Microscope, English name Electron Microscope (EM for short), after more than 50 years of development, it has become indispensable in modern science and technology.

The electron microscope consists of a lens barrel ,

Electron microscopes can be divided into transmission electron microscopes, scanning electron microscopes, reflection electron microscopes, and emission electron microscopes according to their structure and use .

Transmission electron microscopes are often used to observe fine material structures that cannot be resolved with ordinary microscopes; scanning electron microscopes are mainly used to observe the morphology of solid surfaces , and can also be used with

Samples must be pre-processed before viewing biological samples with a transmission electron microscope. Different research methods require scientists to use different treatment methods.

1. Fixed : use as much as possible to preserve samples

   1. In the electron microscope the sample must be in

   Application of electron microscope technology in tumor diagnosis

   Therefore, transmission electron microscopy has broken through the limitation of low resolution of optical microscopy and has become a new tool for diagnosing difficult tumors. Some studies have reported that non-pigmented tumors, eosinophilic tumors, myogenic tumors, soft tissue acinar sarcomas, and neuroendocrine tumors, which are difficult to diagnose with light microscopy, can be clearly diagnosed by electron microscopy. Fine observation of microstructure, looking for differentiation markers of tissue cells, confirming and identifying corresponding tumor types. Apoptosis is closely related to tumors. Electron microscopy plays an important role in the study of apoptosis. Therefore, observing the ultrastructural pathological changes and apoptosis of cells with electron microscopy will provide scientific basis for the diagnosis and treatment of tumors. .

   Application of electron microscopy in tumor differential diagnosis

   The transmission electron microscope observes the structure of tissue cells, biological macromolecules, viruses, bacteria and other structures. It can observe the pathological structure of different diseases and can also identify some tumor diseases. Some studies have reported that the electron microscope technology can distinguish between cancer and Melanoma and sarcoma and adenocarcinoma and mesothelioma; distinguish between thymoma, thymus carcinoid, malignant lymphoma and germ cell tumor; distinguish between neuroblastoma, embryonal rhabdomyosarcoma, Ewing's sarcoma, malignant lymphoma and small Cell carcinoma; distinguishes fibrosarcoma, malignant fibrous histiocytoma, leiomyosarcoma and malignant schwannoma, and distinguishes between spindle cell carcinoma and carcinosarcoma.