What Is Peptide Nucleic Acid?

Peptide nucleic acid, a type of DNA analogue that replaces the sugar phosphate backbone with a polypeptide backbone, is a new nucleic acid sequence-specific reagent that Danish organic chemist Ole Buchardt and biochemist Peter Nielsen began to study in the 1980s. It is based on the first and second generation of antisense reagents. It is a third generation of antisense reagents designed and constructed through computer design and finally artificially synthesized. It is a brand-new DNA analog, that is, a neutral peptide chain amide. The 2-aminoethylglycine bond replaces the pentose phosphate diester bond backbone in the DNA. The rest is the same as DNA. PNA can recognize and bind to DNA or RNA sequences through Watson-Crick base pairing to form a stable double helix. structure.

Peptide nucleic acid

(Chemical term)

Right!

Peptide nucleic acid, a type of DNA analogue that replaces the sugar phosphate backbone with a polypeptide backbone, is a new nucleic acid sequence-specific reagent that Danish organic chemist Ole Buchardt and biochemist Peter Nielsen began to study in the 1980s. It is based on the first and second generation antisense reagents.

(Peptide nucleic acids (PNA)) Because PNA has no negative charge and there is no electrostatic repulsion between DNA and RNA, the stability and specificity of binding are greatly improved; unlike DNA or DNA and RNA hybridization, PNA Hybridization with DNA or RNA is hardly affected by the salt concentration of the hybridization system. The hybridization ability with DNA or RNA molecules is far superior to DNA / DNA or DNA / RNA. It shows high hybridization stability, excellent specific sequence recognition ability, Not be

According to the metabolic stability of PNA, it is mainly used in the field of antisense drugs that inhibit gene expression. Several foreign pharmaceutical and biotechnology companies, ISIS, PE and other companies have invested a lot of energy in research and development. Hybridization stability, PNA is widely used for DNA molecule recognition and manipulation. PNA can be widely used in molecular hybridization, in situ hybridization, mutation analysis, anticancer, antiviral antisense nucleic acid research and application of pathogens and genetic diseases detection. In particular, PNA can replace oligonucleotides for the preparation of gene chips. It will be more stable and specific than ordinary gene chips. It is considered to be an upgraded product of gene chips. PNA can also be used for quantitative PCR, which can be used to detect the amplification reaction of PCR in real time; it can also be made into PNA Beacon for real-time monitoring of RNA expression in cells. With the continuous deepening of PNA basic research and the continuous emergence of new technologies, PNA will show unparalleled superior performance and broader application prospects.