What is a protein electrophoresis?

Electrophoresis protein is a medical procedure used to separate and analyze proteins present in the fluid sample. The sample to be tested is usually a pattern of urine or blood. This technique can also be tested by cerebrospinal fluid.

Separation of protein molecules in electrophoresis is carried out by electric charge. First, protein molecules are treated to provide them with a negative magnetic charge. Then the sample is placed on or in the substrate, usually a kind of gel to which the electric field is applied. Before the invention of electrophoresis gels, the techniques of early proteins of electrophoresis used special types of paper as a substrate.

Due to the charge on the protein molecules, they gradually move towards the positive side of the electric field. Protein molecules are separated by size because smaller molecules travel faster than larger ones. To see the proteins, a dye is usually required.

Serum Pelectrophoresis Rotein is a specific test eLectrophoresis used to identify proteins called globulins that are present in blood serum. Some disorders that can be diagnosed using this test include cirrhosis, liver disease, anemia and a number of different types of cancer. For example, low levels of protein called albumin may indicate liver disease. Haptoglobin and macroglobulin are two proteins that often show reduced levels in certain types of anemia.

Normal urine contains extremely low protein levels. Urine electrophoresis is usually performed after the doctor has diagnosed elevated protein levels in the patient's urine. An example of a protein, which is often present in increased urine, when the patient has a kidney problem, is Bence Jones protein. Gel electrophoresis of different different proteins present in the urine can help identify specific kidney diseases that could cause inYlukovní proteins. Because urine and cerebrospinal fluid tends to have much lower protein levels present than in the case of blood serum, a concentration procedure is usually required before the protein gel electrophoresis is performed on the urine or cerebrospinal fluid electrophoresis.

, as well as its important use in diagnosis, protein electrophoresis is often used to monitor ongoing conditions. Measurement of protein levels that testify to the treated disorder may be an important way to determine whether the disease is progressing and to measure the success or failure of the prescribed drug or treatment. This type of monitoring is usually used for progressive renal and liver disorders and for some autoimmune diseases.