What is the polymorphism of the length of the fragment of the restriction fragment?
polymorphism of the length of the fragment of the restriction fragment, or RFLP, is a change in the homologous sequence of DNA. Polymorphisms are changes in homologous DNA that occur between individuals, while the term restriction concerns the fact that small parts of DNA can be cut out of a large piece by restriction enzymes. Some DNA sections have greater variability than others and the high degree of variability sections are useful in analyzing polymorphism with a restriction fragment.
Polymorphism of restriction fragments relies on the activity of restrictive endonucleases. This enzyme class is found in many bacterial species. In the bacterium, the role of restriction endonucleases is to provide protection against foreign DNA, which obtains access, divided into pieces. In molecular research, restrictive enzymes can be used to fragment DNA at known places in variability in order to analyze the resulting fragments.R sequence and acts to split the DNA molecule at any point where it recognizes the sequence. Bacteria protect svoIn its own DNA before violating a significant pattern of bottom methylation, which prevents the binding of endonuclease restrictions. More than 800 different restrictive endonucleases have been discovered, which together recognize more than 100 unique nucleotide sequences. Most of the recognized sequences are between four and six pairs of bases.
restriction enzymes are used in biotechnology to analyze polymorphism of the length of the restriction fragment for various reasons. Enzymes are used to reduce the length of DNA in certain sequences, so the differences between individuals can be examined. Fragmented DNA solutions, cut in certain well -known nucleotide sequences, are hybridized by probes designed to detect predefined DNA sequences. The samples are then analyzed using gel electrophoresis. This technique creates a gel with multiple dark color strips, each of which represents a different fragment of DNA. By examining the dye pattern, scientists can determine the patternC RFLP present in the sample.
polymorphisms occur widely throughout the human genome, and their analysis has shown that different groups of people tend to share RFLP in common. Some types of RFLP are common for people of different ethnic groups or, for example, geographical origin and connection, for example, there are also family groups. This variation exists throughout the kingdom of animals, making it a useful research tool not only for monitoring human origin, but also for the study of migration and the development of wild animals.
Analysis of polymorphisms of the restriction length of the fragment can provide useful information for clinical and medical purposes. Polymorphism probes Restriction fragments are used in paternal tests, diagnosis of hereditary diseases, forensic science and genotyping. RFLP analyzing patterns throughout the genome are the basis of DNA fingerprints, a technique that supports all these different uses.