What is DNA gel electrophoresis?

DNA Gel electrophoresis is a process used to separate proteins and nucleic acids in molecular biology. The gel usually consists of a crossed polymer and acrylamide that helps in the separation and analysis of various parts of the DNA molecule. DNA Gel electrophoresis is commonly used in the forensic part to determine a specific DNA sequence that helps find the leader of the suspect. This is also commonly used in the genetics and fields of molecular biology and biochemistry. When placed in the electric chamber in the electrophoresis of the gel bottom, each electric charge moves towards the two ends of the chamber. The negative electrical charged molecules move to the positive end of the chamber, while the positive electrical charged molecules move to the negative end. The gel separates each of these molecules so that it spreads evenly while reading. The gel consists of long fibers that prevent molecules moving to help in analyzing individual molecules. Northern blotting is another technique used to identify gene expression in the presence ofthose rna or ribonucleic acids. Eastern blot is used to determine the presence of lipids, proteins and carbohydrates in the DNA sequence. For easy detection of proteins in DNA sequence, the technique of western transmission via gel insulation is used in the electrophoresen chamber.

Zymography is also access to the detection of certain biological functions through the gel DNA electrophoresis. It is mainly used to study enzymes degrading extracellular matrix, which catalyze the destruction of the matrix outside the cell, as well as the basement membrane cells. The use of polyacrylamide gel will help in separating enzymes that are proteins, using a protein substrate such as gelatin or casein. This technique is used to measure the amount of degradation activity found outside the cell.

Electrophoresis

DNA gel is used quite often in forensic. Used to separate blood proteins and DNA found onThe crime scene for determining correlations with available suspects. Gel electrophoresis is also used to determine specific inheritance within genetics because certain DNA and proteins are associated with different races. It is also used to deal with paternity cases to determine the relative relationship between individuals.