What is a microscope of a dark field?

The dark field microscope is a microscope in which the sample appears on the dark background. Dark field microscopy can be used to explore a wide range of samples, from fresh blood cells to live insects. Some microscopes are equipped with a dark field microscopy, while others can be easily equipped with the necessary consumables. Stop blocks the center of the beam light, allowing light to enter only on the sides of the stage. This light hits the sample and scattering, passes into an optical lens and meets the eye of the person using a microscope. Another stop between the stage and the optical lens prevents direct lighting of the eye tightening, causing the background to appear dark because the user can see the scattered light from the sample.

One of the disadvantages of using a dark field microscope is that it requires high intensity light because light levels tend to be low. Extremely bright light can damage some samples, which is concerned in cases where you cannot easily replacet sample. Some aspects of the sample may also be covered in a dark field microscope, such as naturally dark features such as insect veins, and this means that the user could miss some important or interesting details.

The primary advantage of this technique is that it provides high -contrast images. For people who have limited access to expensive microscopic equipment, the use of a microscope of a dark field may be a low -cost alternative that provides useful data for a fraction of the cost. For example, in high school scientific laboratories, students can work with dark field microscopes because the school cannot afford expensive high -contrast microscopes. It is sometimes possible to see very small particles that do not appear in some other types of microscopes.

Science Stores sometimes sells sets of microscopy dark fields that allow people to turn conventional microscopes into micrCunning of dark field. These sets are designed for use with microscopes that have slots for filters and attachments. For microscopes that these properties lack, the dark field effect can still be achieved by recording a round disk, such as a coin or cut -out of the carton to the bottom of the condensing lens.