What is a gram stain?

The gram spot refers to a positive or negative test result of the test that is created when iodine washed into the culture of bacteria to identify its kind. This test, known as a gram of dyeing, works by detecting the presence of lipopolysaccharides (lipoglycans) and peptidoglycans (Mureins) contained in the cell walls of bacteria. Bacteria that have a high level of peptidoglycans are said to be gram-positive. On the other hand, lower levels of peptidoglycans with lipopolysaccharides indicate that the sample is gram -negative. First, a sample of bacteria is placed on a glass and heated only to a point where it is harmless in terms of handler infectious. Furthermore, a sample of bacteria is treated with a solution of Gentian purple-pipe for up to sixty seconds. The slides are then gently rinsed under clean water and applied a gram solution, a mixture of iodine and iodide of potassium diluted in water. This step triggers a reaction to a gentaviolet compound.

Initially, the reaction creates a dark blue color. Follow -upHowever, rinsing with ethyl alcohol leads to color in some samples of bacteria to bleeding, but not in others. The final dye solution is used, which uses a contrasting color, usually a variation of red. The sample that receives this contrasting will seem pink and marked as gram-negative. However, the sample that retains its dark blue color is gram positive.

In addition to identification purposes, the importance of the gram's stain test is that gram -negative bacteria create strong endotoxins that can cause serious diseases such as cholera and Typhoid. Many gram -negative bacteria are also resistant to antibiotics and cannot be made of vaccines. In addition, not all bacteria cause a positive or negative result. In fact, some species are considered to be an adopted or gram variable. Other species are not fully affected by the test simply due to wax protective layer in theircell walls that cannot penetrate the stains.

The

gram of stains was developed at the end of the 18th century by the well -known Danish bacteriologist Hans Christian Gram. However, the original purpose of a gram test was not to distinguish between different bacterial species at all. In fact, Dr. Gram only decided to devise a better way to detect the presence of bacteria in sputum samples provided by patients with pneumonia. It is also interesting to note that the discovery of Dr. Grama, although unintentional, would have a great impact on the study of bacteria resistant to antibiotics half a century later.