What Is a Gram Stain?

Gram Staining is a method used to identify bacteria: this staining method uses the different biochemical properties on the cell wall of bacteria to divide bacteria into two categories, namely Gram Positive and Gram Staining. Gram Negative. This staining method was invented by Danish doctor Hans Christian Gram in 1884. It was originally used to identify the relationship between pneumococcus and Klebsiella pneumoniae. It was later promoted as an important feature of identifying bacteria species. First, it is widely used for clinical diagnosis and treatment of diseases caused by bacterial infections.

Pathogenic bacteria and antibacterial selection

Gram-positive bacteria:

Its important clinical significance lies in: 1. identifying bacteria 2. choosing drugs 3. related to pathogenicity: Gram-positive bacteria can produce exotoxin, Gram-negative bacteria can produce endotoxin; and endotoxin mainly refers to leather Lipopolysaccharides in the cell wall components of Lam-negative bacteria have different pathogenic effects.

False positives and false negatives often occur in experiments. False positives are mainly due to incomplete discoloration, which may be due to excessive smears, or excessive crystal violet staining, resulting in incomplete discoloration. False negative may be due to excessive cell fixation, resulting in changes in cell wall permeability, and false negative results; In addition, the cell culture time is too long, some cells may have died or autolysed, which may also cause cell wall permeability There were false negative results ^[2] .