What is a solid phase extraction?
Fixed phase extraction (SPE) is the name of the process that separates solid and liquid substances in chemical solution. Extraction is most often performed by passing through the sample through the column, which is charged with sorbent, which allows the liquid to pass through the device, while the solid residues are collected above. The purpose of fixed phase extraction is to concentrate and clean samples for further study. These analysts can be contained in solid or liquid mass. If the solid is to be analyzed, it will then be purified by rinsing the solvent.
General extraction of a solid phase uses a disposable cassette similar to a medical syringe. This cassette sits on the top of the vacuum pipe to maximize the speed at which the solvent flows through the column. The cassette contains a C18-Silica that acts as a sorbent and is held in place with frity. The collection tube is located under the SPE column to remove the liquid from the mixture.
The chemist usually uses a four -stage process when performing a SPE. The first step includesLeveling the cassette by wetting the surface with a solvent that is non -polar or only slightly polar. This achieves penetration of the covalently bound phase to start separation.
Second, the column uses water or bumper similar to the nature to the sample used. This liquid wash the whole column. It also saturates the surface of a silica cassette, which could start to dissolve.
In the third step, the actual sample located in the cassette is. The sample passes through the stationary phase of extraction and the analysts begin to interact and remain on the column sorbent material. Liquids, including salts, solvents and various impurities, take place through a column to be collected in the container below. The vacuum apparatus could be employed to facilitate the process.
The last step has a column eluted or mistake again to remove other dirt from the cartridge that contains a sample. Used eluci is a buffer that hasThe correct hydrogen level of potensing (PH) or solvent that is non -polar. The analysis is then ready for further chemical analysis.
Fixed phase extraction is often considered advantageous than liquid extraction, because it takes less time and can be cheaper. SPE is similar to chromatography in that the liquid mixture is issued through a packed column that removes analytes and dirt. The procedure can be done on a large scale by means of a pipe to hold multiple columns to process several samples simultaneously.