What Is an Episome?
An episomal carrier is a substance that allows and assists water-soluble substances to penetrate and pass through the lipid layer of the cell membrane and transfer to cells.
Episomal vector
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- Chinese name
- Episomal vector
- Solid
- Biological term
- Nature
- A protein on the membrane
- Function
- Specific binding to ions on one side of the membrane
- An episomal carrier is a substance that allows and assists water-soluble substances to penetrate and pass through the lipid layer of the cell membrane and transfer to cells.
- An episomal carrier is a substance that allows and assists water-soluble substances to penetrate and pass through the lipid layer of the cell membrane and transfer to cells. It is a protein on the membrane that regulates the active absorption of ions. Specific binding with ions on one side of the membrane forms an unstable carrier-ion complex, and then releases ions on the other side of the membrane, and the carrier returns to the original side. Certain proteins on the cell membrane can Pass certain ions through.
- Construction of a novel Saccharomyces cerevisiae episomal secretion expression vector
- Abstract: The signal peptide sequence (INU) of the inulinase gene of Kluyveromyces is chemically synthesized and inserted into the yeast episomal expression plasmid pYES2 to obtain a new set of secretory expression vectors pYES2I, pYES2II, pYES2, and then PCR Methods The asparaginase gene (ASN) and Bacillus brevis alpha-acetolactate decarboxylase (ALDC) genes were amplified from E. coli, respectively, and ligated to the downstream of the INU to obtain the recombinant plasmids pASN and pALDC. These two recombinant plasmids were transformed respectively. Expression in Saccharomyces cerevisiae strain INVScI, analysis of intracellular and extracellular enzyme activity showed that both ASN and ALDC genes can be secreted and expressed in Saccharomyces cerevisiae, indicating that the inulinase signal peptide sequence can secrete the recombinant protein in Saccharomyces cerevisiae well. Extracellularly, stability analysis showed that recombinant yeast strains were continuously inoculated and cultured for 100 h without selective pressure, and no instability of the recombinant plasmid was found.