What Is Immunoelectrophoresis?

The test result was negative.

Immunoelectrophoresis is a qualitative method for the analysis of antigen composition by combining agar electrophoresis and two-way agar diffusion. Founded by Grabar and Williams in 1953. This technology is a basic immunology technology widely used in the biomedical field because of its high specificity of antigen-antibody reaction and the fast, sensitive and high resolution of electrophoretic separation technology.

Name: Immunoelectrophoresis

category: blood

Normal immunoelectrophoresis

The test result was negative.

Clinical significance of immunoelectrophoresis

Abnormal results: In recent years, this method is mainly used for: analysis of serum protein components, such as multiple myeloma, liver disease, systemic lupus erythematosus, etc .; detection of the purity of antigens and antibodies; research of antibody components. It is also commonly used to detect qualitative and semi-quantitative hepatitis B surface antigen (HBsAg), alpha-fetoprotein, and various immunoglobulins in serum.
People to be checked: those with related diseases and symptoms, especially those with liver disease, and newborns can be tested for prevention.

Precautions for immunoelectrophoresis

Unsuitable: No special requirements.
Contraindications before inspection: Overeating is prohibited, especially spicy food. And taboo strenuous exercise.
Requirements during the examination: fasting blood test. Obey the doctor's request.

Immunoelectrophoresis process

First add the antigen to the small holes in the agar plate for electrophoresis, then dig a horizontal slot in the center of the agar plate, add the known corresponding immune serum, and after the two have diffused with each other for a certain period of time, the optimal ratio of antigen and antibody will be A precipitation arc is formed everywhere. According to the number, location and shape of the precipitation arcs, and referring to the electrophoresis diagram of known antigens and antibodies, the components contained in the sample can be analyzed. This method has a small amount of sample, high specificity and strong resolution. However, the substance being analyzed must be antigenic and the antisera must contain all antibody components.

Immunoelectrophoresis related diseases

Antitrypsin deficiency, pulmonary pleural amoebiasis, parvovirus infection, California encephalitis, multilocular echinococcus disease, meningococcal meningitis, Haemophilus influenzae infection, immune response, hepatitis, shingles virus Scleritis