What Is an Intracytoplasmic Sperm Injection?

Single sperm cytoplasmic microinjection (ICSI) is the use of micromanipulation techniques to inject sperm into the cytoplasm of eggs, fertilize the eggs, culture them to early embryos in vitro, and then place them back into the mother's womb for implantation.

Monosperm cytoplasmic injection

Right!

Single sperm cytoplasmic microinjection (ICSI) is the use of micromanipulation techniques to inject sperm into the cytoplasm of eggs, fertilize the eggs, culture them to early embryos in vitro, and then place them back into the mother's womb for implantation.

1. Severe male infertility (must be confirmed by more than three tests), including oligozoospermia, asthenospermia, teratospermia, and partial azoospermia.

2. Fertilization failure or extremely low fertility rates during treatment.

3 After cryopreservation of sperm or maturation of immature eggs in vitro, ICSI-assisted fertilization is required.

4 Patients with linked diseases, chromosomal balance shifts, and globin production-associated anemia require ICSI for preimplantation diagnostic techniques.

5. Repeated IVF losers.

Pre-treatment check

1. In vitro fertilization and culture transplantation.

2. The man should have an endocrine examination of FSH, LH, and T karyotypes; those with azoospermia should have a testicular or epididymal puncture aspiration test.

3 If there is any genetic infertility caused by hereditary factors, genetic tests should be performed.

1. Same as IVF-ET.

2. If necessary, perform epididymal and testicular sperm extraction techniques (with the participation of a urologist).

3 Requires skilled assisted reproduction micromanipulation techniques. Under the microscope, first adjust the field of vision to the liquid surface containing the sperm, select a normal sperm that is in motion, and brake the sperm. The braked sperm is sucked into the injection, and then the syringe is transferred to the egg-containing liquid. drop. The eggs are fixed with a micro fixation needle. Push the sperm to the tip of the injection needle, and the injection needle passes through the zona pellucida and the cytoplasmic membrane of the egg perpendicular to the 3 o'clock position and enters the cytoplasm. Inject a small amount of cytoplasm with the sperm and as little polyvinylpyrrolidone (PVP) as possible into the cytoplasm carefully. Withdraw the puncture needle and leave the sperm in the cytoplasm. Observe the prokaryote and change the culture medium 16-19 hours after fertilization.

1. Severe chromosomal abnormalities, severe congenital malformations, and severe genetic diseases are not easy to perform ICSI.

2. A second testicular or epididymal puncture should be performed for the first biopsy 4-6 months.

3 ICSI is invasive treatment and is limited to those who need it.

4 It has been confirmed that some genetic diseases can cause oligozoospermia and asthenospermia. Genetic diseases that were eliminated during natural fertilization may be passed on to the next generation through ICSI.

5. Puncture can cause unknown damage to the oocyte. Therefore, pregnancy after ICSI should be followed up with pregnancy and postpartum. ^[1]