What Factors Affect Enzyme Activity?

Enzyme activity is also called enzyme activity, which refers to the ability of an enzyme to catalyze a certain chemical reaction. The amount of enzyme activity can be expressed by the conversion rate of a chemical reaction it catalyzes under certain conditions, that is, the faster the conversion rate catalyzed by the enzyme, the higher the activity of the enzyme; conversely, the slower the rate, the activity of the enzyme The lower. Therefore, the determination of enzyme activity is the determination of the rate of enzymatic conversion. The rate of enzymatic conversion can be expressed as the amount of substrate decrease or the amount of product increase per unit volume per unit time. Enzyme activity can be determined either by quantitatively measuring the change in the number of products or substrates of the enzyme reaction with the reaction time, or by quantitatively determining changes in a property of the enzyme reaction substrate, such as viscosity changes. Enzyme activity is usually measured at the optimum pH and ionic strength of the enzyme and at the specified temperature. ^[1]

Enzyme activity

Generally, the method of measuring the initial speed of the enzymatic reaction is used to determine the vitality, because at this time there are fewer interference factors and the speed remains constant. The unit of reaction rate is concentration / unit time, which can be expressed by the amount of decrease in substrate or increase in product. Because the product concentration varies from scratch, the substrate is often excessive, and the change is not easy to determine, so the product is often used for determination. ^[2]