What Are the Different Types of RNA Isolation Kits?

For the extraction of RNA from various plants, animals and microorganisms, there is an instruction manual in each kit. The experimenter only needs to follow the steps in the instructions. There is no need to prepare other reagents during use, which is very convenient and suitable for rapid RNA extraction. The extracted RNA has good integrity and high purity, and can be used for experiments after molecular biology. However, better kits are more expensive, and the use of kits can be determined in the laboratory according to their own experimental needs.

RNA kit

(Take spin column as an example):

Generally includes:

ingredient	Quantity	stored temperature	Expiration date
Lysate	100ml	2-8	One year
Rinse	30ml	RT	One year
Washing liquid	100ml	RT	One year
RNasefreeddH2O	30ml	RT	One year
RNasefree adsorption column	100	RT	One year
RNasefree collection tube (2ml)	100	RT	One year

In addition, a kit instruction manual is provided. Depending on the manufacturer, different reagents may be provided, such as:

Sample processing

a. Plant tissue: take 100mg tissue fresh or frozen at -70 , grind in liquid nitrogen, add powder to 1ml lysate and mix.

b. Animal tissue: take 100mg of tissue fresh or frozen at -70 plus 1ml of lysate, and homogenize with tissue grinding pestle or homogenizer.

c. Adherent cells: Add lysate directly to the culture plate to lyse the cells, and add 1ml of lysate to every 106 cells. Mix by pipetting with a sampler.

d. Cell suspension: Collect cells by centrifugation. Add 1 ml of lysate to every 106 animal, plant and yeast cells or every 107 bacterial cells and mix.

e. Blood treatment: take 0.2-1ml fresh blood and add 3 times the volume

All related utensil consumables should be RNase-free products. Care must be taken during operation. Wear masks and gloves to avoid contaminating the sample with RNase in the environment.

RNA in water

Of course, in terms of RNA extraction, the extraction effect of the kit is not necessarily very good. In fact, some classic RNA extraction methods are used, and the results are also very good, such as: TRIZOL, EDTA, etc., but the kit is easy to use, the classic method The operation is more complicated.