What is a closer microscope?
The confocal microscope is a special type of fluorescent microscope used to get high quality 2D and 3D images. With the basic (wide) fluorescent microscope, the light of a particular wavelength, it is lit on the sample and the sample absorbs part of this light and reflects the rest at a longer wavelength. Longer wavelength light passes through a dichroic mirror that allows you to go through a longer wavelength light, but not the original (shorter) wavelength light. The person watching the sample only sees the light that has gone through the dichroic mirror. Basic fluorescent microscopes capture both focused and outside the focusing light, which works well for large samples where very high resolution is not required. It does not work so well for viewing fine details. A closer microscope is required to capture high -resolution fluorescent images.
The confocal microscope uses a hole to reject focusing. Instead of going through the light only through the dichroic mirror, the light passesDichroic mirror and hole, which means that a person watching the sample sees only one point of the sample. In order to display the entire sample, the movable mirror scanning light through the rectangular plane of the sample and the computer collects emitted light when the sample is scanned. Complete scanning will lead to optical cut or sample cross -section. This type of microscopy is called confocal laser scanning microscopy, partly because laser is used as a light source.
If you want to create a 3D image, several scans are made and creates several "slices" of the sample and the computer combines each cut and creates a three -dimensional image. The microscope can be programmed to change the space between cuts, depending on how the fresh 3D picture that the user wants to get. The microscope can also change the speed at which the image scans, with slow scanning provides higher resolution than fast scanning.
confocialThe microscope has become a very commonly used device in many life laboratories. They are expensive and somewhat large and often have rooms devoted to their use, because the room must be dark to get a good fluorescent lamp. Although confocal microscopes are most commonly used to view cells and structures in cells, they can be used to obtain an image of any three -dimensional fluorescent sample and sometimes used to view semiconductor materials.