What is Giemsa's stain?
GIEMSA STAIN is a standardized mixture of dyes that clearly excel in different types of cells in the blood paint or thin sketch of tissue. This stain is named for a German chemist, Gustav Giemsa, who first developed it for his work in the study of a parasite that causes malaria - plasmodium . To ensure that the technician that examines the sample can get accurate reading, the steps of the dyeing procedure must be standardized and a mixture of dyes. Giemsa The stain is called a differential spot because it produces different colors depending on what is bound to, such as cytoplasm or DNA.
The Giemsa stain formula has been modified over time to improve the stability of the dyes and colors that are the result. Current standard mixtures include methylene blue, eosin and sometimes azure B. These dyes are often stored in dry powder shape and mixed with water just before use. If water is present in the mixture before use, some compounds will oxidize and stains incorrectly.
The exact steps of the procedure for using the Giemsa stains may vary depending on the organism or type of cell the sample is examined, as well as the composition of the sample itself. The sample, which will be colored using the Giemsa stain, usually blur or attached to the slip very soon after its assembly. The thin blood coating is generally fixed by immersed in methanol, while the thick blood coating is simply left to dry completely at room temperature. The slides are then soaked in the stain for time and then rinsed with water that has a neutral pH. They can dry the slides before watching.
As a result of differential coloring produced by Giemsa stains, plasmodium cytoplasm spots light blue, while DNA seems to be red or purple. Another parasite, giardia lamblia, is a tinted pink purple, except for DNA, which spots very dark blue. histoplasma capsulatum, sponge, is found in its yeast form in human white blood cells and dark blue spots.
This coloring process is also useful in chromosome studies and visualizing differences between different blood cells. Chromosome spots very dark blue in some sections and light blue in others. This causes the strip effect that helps genetics to find places where chromosomes have undergone unusual changes. Red blood cells of pink spots, while granules in mast cells show like purple spots. White blood cells color different shades of blue, allowing different types - basophils, eosinophils, neutrophils and others - to distinguish from each other.