What Is the Giemsa Stain?
The principle and result of dyeing are basically the same as those of Reiter's staining method. However, this method has a better coloring on the nucleus and parasites, and the structure is more clear, while the cytoplasm and neutral particles are less colored. In order to take into account the strengths of the two, composite staining can be used. That is, dilute the Giemsa solution instead of the buffer solution, and stain it for 10 minutes according to the Rieter staining method, or first stain it with the Rieter staining method and then counterstain with the diluted Giemsa.
Giemsa staining
Right!
- The principle and result of dyeing are basically the same as those of Reiter's staining method. However, this method has a better coloring on the nucleus and parasites, and the structure is more clear, while the cytoplasm and neutral particles are less colored. In order to take into account the strengths of the two, composite staining can be used. Use diluted Giemsa solution instead of buffer solution.
- Giemsa staining
- Giemsa Dye Solution by Azure
- Wright
- Preparation of blood smears and cell staining Microscopy of blood smears is the basic method of blood cytology examination. It is widely used in clinical practice, especially for the diagnosis of various blood diseases. It is widely used in blood cell analyzers The observation of blood smears can also be used as a simple method to judge the results of the instrument. Observing the number of white blood cells and platelets in 10 high-power field blood smears compared with the table roughly estimates the number of these cells in the blood, which serves as a reference for quality control after the analysis of the results of the instrument. However, the poor preparation and staining of backlog smears often make it difficult to identify cells and even lead to incorrect conclusions. For example, cells with excessively thick blood membranes overlap and shrink, cells with too thin blood membranes are mostly concentrated at the edges, and the cells are unevenly distributed; acidic or alkaline staining can cause abnormal cell staining reactions. Therefore, the preparation of blood smears with appropriate thickness, uniform distribution, and good staining is one of the important leather techniques for hematological examination.
- In order to observe the internal structure of cells and identify various cells and their abnormal changes, blood smears must be mocked and stained. Most of the staining methods of blood smears are derived from Roche staining. Riet staining is commonly used. [1]