What Are the Different Methods of Fetal Paternity Testing?

DNA paternity test is to use forensic science, biology and genetics theories and technologies to analyze the genetic characteristics from the similarity in the morphological structure or physiological functions of the offspring and parents, to determine whether the parents and children are biologically related. DNA paternity test: Also called paternity test, it is an important part of forensic medical evidence test.

dna paternity test

DNA identification is most commonly used to identify parent-child relationships. Human blood, hair, saliva, oral cells, and bones can be used for paternity testing, which is very convenient.

There are mainly three types of paternity tests. One is the family in crisis, and the husband and wife are in conflict. The second is the single parent test, which is mainly because the man suspects that the child came with the child alone. Relevant regulations must be married and have children in the Mainland

Currently, the most commonly used method for identifying parent-child relationships is DNA typing. Human blood, hair, saliva, oral cells, etc. can be used for paternity testing, which is very convenient. A person has 23 pairs (46) chromosomes, and a pair of genes at the same position on the same pair is called

DNA paternity test

DNA paternity testing steps are as follows:

Step 1: DNA extraction

The DNA contained in the nucleus of the sample is extracted and then purified to remove impurities from the sample.

Step 2: PCR amplification

The Chinese name of PCR is polymerase chain reaction. To put it simply, the step of PCR amplification is to make a large number of copies of the fragments we need through an enzymatic reaction on a PCR instrument, and enlarge it to be seen by some special instruments. Degree.

Step 3: Post-PCR reaction

This step is mainly on the preparation stage of the sequencer test. The double-stranded DNA is opened, and some internal standards for detection are added, which are mainly used to mark the length of the detected fragments.

Step 4: Capillary Sequencer Detection

Due to the charge of DNA, the electrophoresis speed of different fragments of DNA is different by capillary electrophoresis. At the same voltage and the same electrophoresis time, the swimming distance is different. These different lengths can be measured by the internal standard added earlier. It can be distinguished and displayed on the computer at the same time by a certain software, which is convenient for inspectors to process and analyze the data.

Step 5: Analyze the data and issue a report

The main purpose is that the tester analyzes, summarizes, and calculates the results, and then draws out conclusions and reports.

DNA paternity typing map