What are the different methods of nicotine detection?

Life or Health Insurance Application will often include nicotine detection test. Chromatography with fluid fluid can detect nicotine - or its primary metabolite, kotinin - in urine, blood, saliva and hair. Measurement of chicken in the urine is considered to be the most sensitive technique of nicotine detection. None of these tests will perfectly distinguish between active smokers, passive or other hands and non -smokers, because the individual differences in nicotine metabolism are very different. However, nicotine detection testing will provide a good estimate of the total nicotine exposure. Chewing or inhalation of tobacco introduces nicotine into the body, where the liver is metabolised and excreted in the urine. The primary metabolite of nicotine is kotinin and kotinin is a primary method of nicotine detection because it has a half -life up to 10 times if nicotine. In other words, it is stable in the body much longer. If nicotine overdose is suspected, such as nicotine rubber, nicotine is measured instead of cotinin.

nicotine detection using hair samples is rare outside experimental tests because it is expensive. However, hair testing can evaluate long -term tobacco use as it can detect nicotine exposure up to 10 days before the sample. Slive nicotine tests are simple and non -invasive; The saliva is absorbed from the mouth by a sample of a substance or swab, but it may be difficult to get a sufficiently large sample. Children or adults with dry mouth may not produce enough saliva for the test sample. Salt tests also rely on a very recent exposition of nicotine and may fail to distinguish light active smokers from passive smokers.

Blood samples allow you to detect chicken in blood plasma, although blood sample requires Great solves more processing than urine or saliva samples. The blood sample is first centrifuged to separate the blood cells from the plasma, and the heavy proteins are then precipitated. The pipe tube is a second timeDěna and then placed in the evaporator to remove the remaining liquid; Dry proteins, including kotinin, are dissolved by methyl alcohol. Once again, the tube is centrifuged to separate the remaining heavy proteins, and the liquid is poured and is used for analysis. This process is more time -consuming and more expensive than urine or saliva tests.

nicotine detection with urine is very sensitive and detects even small levels of second -hand smoke. It is the most common method used by insurance companies to distinguish active smokers from passive smokers. Whether nicotine detection is urine, saliva or blood serum, you can see the difference between people who actively smoke three or more cigarettes a day and the high levels of the other ha smoke. Heavy smokers can also be distinguished from light smokers with a quantity of cotinin in the sample.