What Are Human Cell Lines?

Cultures with special properties or markers obtained from primary cultures or cell lines by selection or clone formation are called cell strains. ^[1]

A cell line is a cell population formed by single cell proliferation by single cell isolation and culture or screening. The special nature or signature of the cell line must be present throughout the culture.

Cell line

Primary cultured cells are generally difficult to pass down to about 10 generations, and cell growth will stagnate, and most cells will age and die. However, there are very few cells that can survive the "crisis" and continue to pass on. These surviving cells can generally pass to the 40-50 generation. Such passage cells are called cell lines.

Cell line

The genetic material of the cell line has not changed. When the cells reach the 50th generation, there will be a "crisis" and they cannot be passed on. However, the genetic material of some cells has changed and has the characteristics of being cancerous. It is possible to pass them on indefinitely under culture conditions. Such passage cells are called cell lines.

Cell line

The primary culture is called CellLine after the first passaging, so the cell line can refer to the cells that may be generally passaged. The cells that can be continuously passaged are called continuous cell lines or infinite cell lines, and those that cannot be continuously cultured are called limited cell lines. Most diploid cells are limited cell lines. Cultures with special properties or markers obtained from primary cultures or cell lines by selection or clone formation are called cell strains, that is, cell lines are cultured by single cell isolation or screened Method, a cell population formed by single cell proliferation. The special nature or signature of the cell line must be present throughout the culture.