What is a fluorescent microscope?

Fluorescent microscope is a microscope that is used to explore samples with luminescent properties or samples prepared with substances that create luminescent properties. In this type of microscopy, the sample itself is a light source. Using a fluorescent microscope, a large amount of information can be collected and these microscopes can also be used to create stunning images in which detailed structures are clearly visible. These microscopes use the properties of certain chemical compounds. When these chemicals are lit by the light of the right wavelength, the classic ultraviolet light. If luminescence is short, it is known as fluorescence, while the extended luminescence time after excitation is called phosphorescence. In both cases, the color of luminescence will differ depending on the chemicals involved, as well as the exact length of the luminescence period.

In fluorescence microscope, light of a particular wavelength passes through a specialized microscopicA capacitor that focuses light into a very narrow beam. When the light hits the sample, luminescent compounds are excited and begin to emit light. Using a dichroic mirror that filters out the beam of light used to excite the pattern, the scientist can clearly see luminescence and make notes about his properties, or photograph a sample in the microscope phase for the future link.

Since the light used in a fluorescent microscope is often potentially dangerous to the eyes, it is usually necessary to use the polarizing filter on the piece of the eye so that the user's eyes are not damaged by the microscope. Polarizing filters can also be used to correate color or increase contrast, so luminescence is clearly visible. As with other microscopes, clarostobras can be adjusted by focusing the microscope components and the magnification level can also be increased or decreased as needed.

in some caseH can be naturally luminescent, as in some minerals that will be fluoresces or phosphores in the light of a certain wavelength. Samples can also be marked with molecules known as fluorofors. These molecules may target specific structures in the sample and create a fluorescent image of the notes structures when the sample is excited by the light in the microscope.

fluorescent microscope can be a costly purchase, especially in the case of high quality microscopes. In general, this type of microscopy is only available for very well -equipped laboratories.