What is the northern blot?

Hybridization of Northern Blot is a technique that is used to detect the levels of messenger RNA (MRNA) in the sample and quantification the amount of MRNA that is present. Although there are other stronger techniques, Northern Blot remains standard for its simplicity and easy use. The northern crap technique also allows a direct comparison of multiple samples in one experiment.

Messenger RNA is a type of nucleic acid that is produced when expressing the gene. The gene that is on and functional in the cell is rewritten to MRNA. MRNA is then transported to a cell cytoplasm, where it is translated into a protein. Analysis Northern Blot Analysis of the cell MRNA sample can therefore provide useful information about a number of proteins produced by the cell. MRNA samples of known sizes are performed simultaneously to ensure control. After the electrophoresis is complete, the samples are transferred and immobilized on a solid membrane, usually made of materials such as nylon. Samples are then exposed to a radioactively marked probeIm for several hours before analysis. During the analysis, MRNA, which has been hybridized to a radioactive marked probe, can be clearly detected by X -ray.

The northern fuss technique allows you to observe the formulas of gene expression between different types of tissues. Messenger RNA extracted from cells of various tissues and organs can be compared and cellular reactions to stress, infection and many other stimuli can be examined. Looking at the differences in MRNA expression under different conditions, the scientist can determine what types of proteins that the cell produces in response to certain stimuli. This may provide traces of the functions of unknown proteins or in the case of known proteins information about cellular reactions to the stimuli in question.

For example, the North Blot may show that the expression of unknown protein increases in response to the presence of a specific toxin. Then it could be theorized that the protein is involved in reducing toxicity, perhaps by transportchemicals from a cell or converted to a metabolite. If known proteins are examined, the documentation of protein expression changes can provide information about the response itself. If the expression of a well -known molecular transporter has been increased, for example, it can be confirmed that the cellular reaction to toxin should transport toxin from the cell.