What is a complicated plasmid?
Plasmid is a circular piece of bottom that is found in many bacteria. The most important feature of plasmids is that they are replicated independently of the main host DNA. Plasmide is often used in the technology of recombinant cloning to cloning newly insulated genes. It is also very common to use a complicated plasmide to express a large amount of a known gene to obtain or protein from it. Such expression of the recombinant gene was essential for the biotechnology industry. Many other types of bacteria can hide such plasmids. These pieces of DNA self -being can naturally transmit between different types of bacteria. Yet it was sometimes difficult to introduce recombinant plasmids into other bacteria species.
The primary procedure for introducing DNA into other cells is called a transformation in which bacteria are treated with chemicals that create more likely to attract foreign DNA. Another technique includes shocking bacteria with electric shock. This is known as electroporation.
reasons for theThe boost of the recombinant plasmide varies. When DNA is first insulated from a certain tissue or organism, it is transformed into plasmids to form a library. Then the DNA can be extract from the individual colonies. Furthermore, they can be proven by DNA sequencing to determine what types of genes are present when sequences are present in the database. Sometimes genes with unknown functions are cloned.
In other cases, the gene product is well known, but scientists want to express a large amount for further study. The gene can be cloned into the recombinant plasmids that are excessively expression vectors. They are designed mainly for the production of large amounts of RNA or protein. This was particularly valuable to the recombinant man of theproteins, which were previously often available only in the dead, making the study of a particular gene difficult.
In the construction of a plasmide that can be used in molecular cloning, n is connected na number of factors. Plasmid must have an optional brand. This allows you to choose a cell with a gene. Normally, cells' populations lacking a marker gene produce a number of cells that carry it. Generally, a complicated plasmide has resistance to antibiotics or may grow in the absence of a particular amino acid.
Such a plasmid needs replication, so it can start synthesizing its recombinant DNA. In addition, the recombinant plasmid requires a set of special sequences to allow the restriction enzyme to break down DNA to allow the gene inserting into the cloning vector. There are a large number of restrictive enzymes that are highly specialized in specific DNA sequences, which must be present where Gene's strength and end are.
Traditional tribes of bacteria have been used for cloning DNA for decades. In addition, there are new sets that use specially designed bacterial strains to facilitate excessive expression of gene product. Combine technology for cloning gene with methodsOU, which allows easy cleaning of protein expressed from the gene as soon as it was cloned into the recombinant plasmide.