What is antibody detection?

Antibodies detection is important in medicine for disease detection because antibodies are large proteins that can detect viruses and bacteria. Detection of antibodies is carried out in laboratories and in the terrain for investigating antibodies and antigen bonds and identifying the antibody by its specific color change when it is associated with an enzyme reacting substrate molecule while still bound to antigen. The detection test is known as an immunosorbent test associated with the enzyme (ELISA). Before the development of Elisa, the only tests of radioimunoanalysis were that identified antibodies with their specific radioactivity at the age of 60. At the beginning of the 70s, several ELISA techniques have been developed. Then a primary antibody from a patient who binds with antigen is introduced. Further, a secondary antibody with the connected enzymemje is introduced and added to the enzyme substrate. This causes a reaction that changes the color of the enzyme, signaling two antibodies combined if the antibody is positive from the patientfor this antigen of the disease. The power of color change indicates the amount of diseases that the antibody has detected; This color force is then quantitatively measured by the spectrometer.

Another ELISA technique, when patients are gross or unclean, is a competing Elisa when an unmarked antibody is incubated with a patient's patient's patient. The Bound antibody/antigen complex is washed so that the unbound antibody is removed and the secondary antibody associated with the enzyme specific to the primary antibody that binds with the antigen is introduced. When adding a substrate to the enzyme, if the disease is detected by an antibody, the enzyme creates a chromogenic or fluorescent P Properties as a signal. The field test used by Israeli doctors uses a tube full of fragments of proteins and antibodies and the introduction of blue liquid. If the blue liquid turns red within ten minutes, a particular antigen in a tube isAntibody recognized for this particular disease.

In the field of difficult to address areas of developing countries, laboratories are not easily accessible. Many commercial antibodies detection sets have been sold for healthcare professionals to test tuberculosis, especially tuberculosis of virus or autoimmune deficiency for pediatric and human immunodeficiency. One study containing 68 specific studies found that these sets were not as reliable as sputum microscopy blurred.

Malaria is another disease for which the detection of antibodies uses a fluorescent antibody test. This procedure is good for screening of blood donors, because transfusion malaria may not be manifested in a simple blood examination. If the patient commands repeated samples of negative blood, but shows a strong symptomology of the disease, the test of fluorescent markers can detect it. When examining under a microscope antibody for malaria they often show the color of the apple-green that is for the parasite mAlaria positive.