What Is Quantitative Fluorescence?

Fluorescence quantification uses fluorescent dyes or fluorescently-labeled specific probes to track the PCR products, monitor the reaction process online in real time, and analyze the products with the corresponding software to calculate the initial concentration of the sample template to be tested.

Fluorescence quantification

Fluorescence quantitative pass

1. The specificity of the amplification must be determined;

2. Only with the same goal

1.

Sample materials such as cells (10), tissues (300mg), blood (1ml), leaves (100mg), genomic DNA or total RNA (volume 20l, concentration 50 ng / l).

Primers and probes and sequences, reverse transcription gel maps, raw data (including amplification curves and melting curves), data analysis results and experimental reports.