What is quantitative culture?
Quantitative culture is a term belonging to microbiology. It describes a number of techniques that microbiologists use to count, how many microbes are present in a particular sample. Some analytical techniques only identify the presence of certain microorganisms, not the number of microbes present, and therefore these methods are not quantitative.
When a microbiologist provides microbes enough nutrients for growth and multiplication, this process is called cultivation. Microbes are very small and can be present in large numbers in samples. It is virtually impossible for a microbiologist to know what is in the sample, and at what concentrations without the microorganisms growing at easy -to -read levels.
Microbiological samples can be anything that could contain microbes. Examples include food, blood or water. Some of these samples most often contain a lot of microbes such as minced meat, or have little or no microbes like blood. The ST Analysis is to place a sample in the growth medium PRO culture that can show how many individual microbes are present.
The analyst usually removes a specific sample rate such as 1 ml of water. It is that it can find out how many microbes are in this ml and thus calculate how many microbes are present in the sample as a whole or in the sample source. If they think that the total microbial number in this ml sample will be high, the analyst performs dilution to the point where the result is at levels that are not too high to read.
One method of quantitative culture includes ml sample or diluted with nutrients in a solid medium. Then the analyst incubate at a specific temperature and time to reproduce the microbes. From each initial microbes present in the sample, which can grow under these conditions, one medium is present isl is present a blob of many microbial cells called colony. The analyst then simply calculates them and, if necessary, the number ofIt is diluted to see how many microorganisms are present in the initial ml of the sample.
Knowing how many microbes are present in the sample, through quantitative culture, it is a useful indication of the purity of the sample. For example, urine in healthy people should not have any microbial contamination and higher levels of contamination indicate the intensity of infection. Through quantitative culture, the microbiologist can not only find out how many microorganisms are in the sample, but also how many certain individual species. To do this, the sample must mix with special nutrient media, which only grow certain types of microbes, instead of general media, which allows the growth of many microbes.
Some microbiological techniques allow the growth of existing microbes before testing. Since the initial amount of microorganisms in the sample multiplies, the number is growing. These methods of microbiological analysis, although they can help identify the existence of specific microorganisms in the sample, cannot accurately calculateT levels of microbes and are not part of quantitative culture.