What is the test fixation test?
Complement fixation test is an immunological medical test that detects specific antibodies in the person's blood. This test was once used as a means of diagnosing infectious diseases, but nowadays, polymerase chain reaction and other DNA detection methods are commonly used. The value of the add -on fixation test now consists in its ability to diagnose autoimmune disorders or monitor certain chronic conditions.
Complement proteins are proteins involved in an immunological reaction called complement cascade. The process called opsonization requires the presence of antibodies specific to proteins on the surface of bacteria. Proteins in a complement cascade can also directly kill bacteria in the molecules that cause rupture, something that may occur in the absence of specific antibodies.
Sometimes a doctor orders a test to fix supplements for a patient who is experiencing repeated infections. A test sample is a small amount of blood, usually taken from the vein of the arm, without separatePreparation for the patient. After submission of the sample, it is processed to separate the liquid serum from the blood that has been left to coagulate. The accessories fixation test is then performed for serum.
Before performing the test, some further processing is required to destroy the patient's own supplementary proteins. This is necessary because the test fixation test measures the speed at which the patient's antibodies respond to complement and concentration of supplements and the level of activity differs from man to person. In order to achieve standardized results, the patient's supplement is destroyed and the complement to this is used by known concentration and level of activity.
At this stage, the accessory sample is then ready for testing and interest is added to the test sample. The antigen is specific to a particular type of pathogen or for autoimmune antibodies. The red blood cells are then added to the sera sample, bound to the antibodies specific tocells.
If the sample contains antibodies specific to the antigen tested, the antibodies will respond with the addition that has been added. This causes all the supplement in the sample to be used and nothing reacts with sheep cells in the sample; However, if specific antibodies are not present, the supplement will not be used. In this case, this supplement causes the red blood cells to burst, which turns the pink test sample. Therefore, if the sample turns into pink, this is a negative result and if the sample remains clear, it is a positive result.
AdDs for this test is the monitoring of a person who has a known autoimmune disease. In this case, the levels of testing activity may be specific to antigen-specific refilling in a good way to measure the effectiveness of a certain treatment. This is often done in people with systemic lupus erythematosus (SLE), autoimmune disease that develops when the immune system of the body reacts to proteins in cell nuclei. Test Fixation DOPLňka is also useful for the diagnosis of central nervous system infections, as the test does not require a sample of cerebrospinal fluid that is much more difficult to obtain.