What is an Anaerobe?

Anaerobic bacteria are a group of bacteria that grow better under anaerobic conditions than in aerobic environments, but cannot grow on the surface of solid media in air (18% oxygen) and / or 10% carbon dioxide Bacteria. This kind of bacteria lacks a complete metabolic enzyme system, and its energy metabolism is carried out by anaerobic fermentation. It can cause infections in different parts of the body, including appendicitis, cholecystitis, otitis media, oral infections, endocarditis, endometritis, brain abscesses, myocardial necrosis, osteomyelitis, peritonitis, empyema, salpingitis, septic joints Inflammation, liver abscess, sinusitis, wound infection after bowel surgery or trauma, pelvic inflammatory disease, and bacteremia.

Anaerobic bacteria

Anaerobic bacteria are a group of bacteria that grow better in anaerobic conditions than in aerobic environments, but cannot be grown in air (18% oxygen) and / or 10% carbon dioxide.

Anaerobic bacteria has no accepted exact definition, but it is generally considered to be a type that can only grow under low oxygen partial pressure conditions, and cannot be solid cultured in air (18% oxygen) and / or 10% carbon dioxide concentration Bacteria growing on the basal surface. According to their tolerance to oxygen, they can be divided into

According to the tolerance to O, anaerobic bacteria can be divided into three categories:

(1) Anaerobic bacteria that are extremely sensitive to oxygen: the representative species is Moneromonas. These bacteria have high requirements for anaerobic conditions. They die when exposed to air for 10 minutes, which is difficult to isolate clinically.

(2) Moderate anaerobic bacteria: the representative strains are

Ubiquitous

Anaerobic bacteria are human

At present, there are many methods to identify anaerobic / microaerobic bacteria, but the culture identification method is always the most reliable and accurate identification method.

Anaerobic / microaerobic culture equipment

Anaerobic culture equipment includes: anaerobic bag, anaerobic tank and anaerobic workstation

Micro aerobic culture equipment includes: micro aerobic tank and micro aerobic workstation

As anaerobic bags and anaerobic tanks (micro-aerobic tanks) can only provide the corresponding gas environment for the samples in the culture stage, they cannot provide the gas environment in the operation stage. Due to inconvenience in operation and high cost of consumables, they gradually tend Eliminated. And anaerobic workstations (micro-aerobic workstations) that can operate and cultivate the whole process have become more and more necessary equipment for anaerobic / micro-aerobic culture.

Generally speaking, the anaerobic workstations and micro-aerobic workstations of each manufacturing brand (including domestic and imported) are independent models. The anaerobic workstations cannot perform micro-aerobic culture, and the micro-aerobic workstations cannot perform anaerobic culture. This brings high cost of procurement and use to users of microbiology laboratories, and also makes the already crowded microbiology laboratories more space crisis.

The British Electrotek anaerobic / micro-aerobic workstation has achieved one box of dual-use for the first time, which can be anaerobic and micro-aerobic, and has become the front-line representative of this equipment technology.

Anaerobic / microaerobic media

Medium for anaerobic and microaerobic cultures.

According to different uses, it is mainly divided into: bacterial growth medium, selective medium and identification medium.

At present, the experimental materials have generally been domestically produced, and only a few parts of the professional field need to use imported brands.