What is a conjugate of antibodies?
The antibody conjugate is a marker molecule that can be used in histological or enzymatic detection tests for locating bound antibodies. It works by physically connected or conjugated to the antibody that is used to indicate an antigen of interest. An antibody that is marked on the conjugate of antibodies is a tool that is often used in research to identify the exact location or amount of proteins or antigens of interest against antibodies or antibodies. By exposing an antigen or protein that is interesting on the antibody that has been against it, antigens or proteins can be described. Using the antibody conjugate - ie an antibody that has been conjugated with a molecule specifically used or synthesized for easily detected - antibodies on their respective antigens can then be identified and tested. The most common type of antibody conjugate is a fluorescent molecule that allows you to identify antigens in tissue under fluorescewith some microscope. Other conjugates include molecules such as horse radish peroxidase, which is used in conjugation with antibodies for later development with diaminobenzidine (DAB).
The primary antibodies will often be caused against specific antigens, while secondary antibodies will be conjugated on the marking of molecules and increased against the primary antibody. As an example, one might be interested in identifying an antigen called antigen X. One would then use an antibody raised in a mouse that reacts and adheres to Antigen X. In order to find these primary antibodies and find these antigen x molecules. The inscription, which makes this secondary antibody detectable in enzymatic or fluorescent microscopic tests. PRO tests in antigens localization using an electron microscope are often used as a conjugate of antibodies as they can be detected by electron beam.
The reason for the use of primary and secondary antibodies, instead of using the conjugation of the antibody in which the primary antibody is directly conjugated, is cost efficiency. The conjugation of antibodies is expensive in terms of time and reagents. Especially for unusual antigens do not stand conjugation of antibodies on primary antibodies. Rather, by conjugation of antibodies on secondary antibodies, a large amount of conjugated antibodies can be synthesized at the same time and used in tests with a number of primary antibodies adapted to locate many different antigens, so the production process is useful and cost -effective.
In some cases, however, the primary antibody will reduce the noise in the detection signal as a result of increased non -specific binding of the antibody that is interrogationDEK USE MORE ACCUMENTS. Although these expensive, highly sensitive tests will still require this method. It is possible to buy sets that allow the researcher to mark conjugates with their own antibody, increase the costs of the agent, but reduce the steps of the protocol and possibly increase the ratio of the signal-shum in the final test of antibodies.