What is a chromosome walk?

Chromosome walking is a method of position cloning used to find, isolation and cloning a particular allele in the gene library. The allele is a gene for a particular genetic property transmitted from adults to their offspring, such as the allele for brown eyes in the eye color gene. Sometimes the approximate location of one allele in the chain deoxyribonucleic acid (DNA) may be known. To isolation of a particular allele for a genetically transmitted disease, chromosome walking can explore the desired sample in an unmapped DNA sequence outside the previously mapped sequences.

For locating a particular gene for disease, walking begins in the nearest gene that has already been identified, known as the marker gene. Each subsequent sequence gene is repeatedly tested for what is called overlapping and mapped for their accurate sequence location. Finally, the Walk genes reaches the mutant gene in an uncharted sequence that binds to the fragmentgen of this particular disease. Once the gene is cloned, it mayt its function fully identified. During this process, tests are performed to fully identify the properties of each clone to make their location for future use.

There are almost half a dozen of position cloning tests that are performed before a chromosome walk to narrow the specific genetic sequences that may contain the required mutant gene for the disease. Once markers are found on both sides of the heartless probable sequences, the chromosome walk can start from one of the markers. Testing on every consecutive clone is difficult, time consuming and changes according to species. There are different tests for plant -related genes than in genes involved in the human gene library.

position cloning uses genetic markers known to inhabit the chromosomes of individuals who have specific diseases. I know these databasesThe common features allow testing that can be used to identify individuals who may or may not have certain recessive genes for diseases that have not yet submitted. Chromosome walking, along with all other tests performed before chromosome walking, requires exceptionally well -equipped laboratories in which all phases of cloning, tests and analysis of found mutations can be performed.