What Is Fructosamine?

Fructosamine is a substance formed during the non-enzymatic saccharification of glucose in plasma. Since the half-life of plasma proteins is 17 days, fructosamine reflects the blood glucose level within 2-3 weeks. The amount of fructosamine depends on blood glucose. Concentration level.

Fructosamine is a protein and glucose nonenzyme in plasma
Fructosamine is a substance formed during the non-enzymatic saccharification of glucose in plasma. Since the half-life of plasma proteins is 17 days, fructosamine reflects the average blood glucose level within 2 to 3 weeks. The fast and cheap determination of fructosamine is an indicator to evaluate the control of diabetes, especially for fragile diabetes and gestational diabetes with large blood sugar fluctuations. It is more practical to know the average blood glucose level.
Glycated hemoglobin reflects the average blood glucose level 2 to 3 months before the measurement. It can be used as an objective long-term control indicator of diabetes and a reference for the diagnosis of mild diabetes. Both are unaffected by each meal and cannot be used to directly guide daily insulin dosages [2]

Fructosamine measurement principle

Principle Serum fructosamine reacts with nitrotetrazolium blue under alkaline conditions to produce a purple-red compound. The color is measured at a wavelength of 550 mm, the OD value is measured, and the content can be obtained by comparing with the DMF standard.

Fructosamine measurement reagent

1. Chromogenic reagent: Weigh 100mg of nitroblue tetrazolium chloride (NBT), dissolve and dilute to 1L with 0.1mol / L carbonate buffer with pH value 10.8, store in refrigerator, save approximately Stable for half a year.
2. 0.1molL of carbonate buffer, pH 10.8, weigh 9.54g of anhydrous sodium carbonate and 0.84g of sodium bicarbonate, dissolve in distilled water to 1L.
3, 4 mmol / L DMF standard solution: Weigh 99.6mg of dimethylformamide (DMF, dimethylformamide), dissolve in 40g / L bovine serum albumin and make it to 100ml. [3]

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