What Is a Microtome?

The microtome is a machine for cutting thin and uniform tissue pieces. The tissue is supported by hard paraffin or other materials. Each slice is automatically advanced forward (toward the knife) by the slice thickness device. The gradient of the thickness device is usually 1 micron. When paraffin-embedded tissue is cut, multiple sections are made because they adhere to the wax edge of the previous section.

Chinese name: Slicer
Foreign name: microtome

Belongs to: Machinery Automation
Function: Cut thin and even tissue pieces
Application: Medicinal materials, food, cell tissue, wax, etc.

Slicer Abstract

A microtome

Machine for making cell or tissue sections. Rotary and slide-away microtome for optical microscope. For the electron microscope, there are ultra-thin microtome, which use glass knife or diamond knife to make ultra-thin section. Material devices are mechanically propelled and thermally expanded. Frozen microtome, cryostat, and frozen ultra-thin microtome are used for frozen section method. A cylinder microtome is used for free hand slicing. ^[1]

Reference: Section [1] In order to facilitate the observation of the cell tissue with an optical microscope and an electron microscope, after fixation, it must be cut into thin slices that can pass light and electron rays. This is called a section. [2] slice = tissue slice.

Second, the pulp and paper industry: the general form has a knife disc slicer, the heavy knife disc also plays the role of a flywheel to stabilize the slice process. The blade is installed along the disc surface in a radial pattern and protrudes about 20mm. The log is fed into one side of the turntable through a chute. The blade is sliced at an angle to the log axis. The processing capacity is 290m3SUB / h. Generally, the length of the wood chip is 24mm and the diameter of the blade is 3360mm , The cutter head rotates at 249 rpm, there are 16 blades installed on the cutter head, driven by 4 motors, the total power is 4 × 315kw, and the motor speed is 25 rps.

Three chip cutter

Device for cutting polymer strips into pellets. The slicer consists of a guide plate, a feeding roller, a pressure roller, and a rotary cutter head. The cutter head is driven by a continuously variable transmission. The feed roller is driven by the cutter head through a set of changing gears. The cutter head is equipped with multiple blades according to the size of the pellets. Changing the change gear can change the cutting length. Adjusting the continuously variable transmission can change the unique speed of the belt. The slices are cylindrical or rectangular. Most use underwater pelletizers. The advantage is that it can avoid the contact of the melt or the slice with the oxygen in the air, make the slice smooth, and eliminate the powder caused during granulation.

Use of slicer

The main use comparison of the microtome is to slice the materials such as medicinal materials, food, cell tissue and wax to facilitate their next process. Its main uses are as follows:

Food slicer

The slicers used in the food industry are: red date slices; hawthorn slices and other commonly used slicers

Slicer

The food slicer is suitable for cutting boneless meats and other elastic foods like mustard.

The material is cut into meat slices, etc.The machine has a compact structure, beautiful appearance, easy operation, high efficiency,

Low power consumption, easy to clean and maintain, safe and hygienic, evenly cut meat and automatically roll.

Imported Italian blades and belts with unique automatic lubrication device, powerful,

It is an indispensable meat processing machine for hotels, restaurants, canteens, meat processing plants and other units.

Machine. The slicers used in the catering industry are: beef and mutton slices, etc. These are common in the market

Of some food slicers.

Slicer for medicinal herbs

For example, the ginseng slicer is specially designed for ginseng, pharmacy, clinic, and family to cut American ginseng, velvet antler, gastrodia and other ginseng drugs. It can be cut into oblique slices, horizontal slices, straight slices, uniform thickness and beautiful color. The cutter head is equipped with four blades, which can be adjusted arbitrarily. It has the advantages of small size, convenient use, beautiful slicing, simple operation, stable quality and long service life. Suitable for ginseng, plant rhizomes and other noble medicinal materials. ^[2]

See below for specific references

Microtome

Pathological slicer: R139 type rotary paraffin slicer

Main performance indicators:

1. The streamlined cover does not need to be disassembled and is easy to clean; the removable tray on the top can hold specimen boxes and other objects.

2. The sample clamping system can be adjusted arbitrarily on the three-dimensional axis, which is convenient to use.

3. Completely eliminate the phenomenon of knife skipping, uneven thick books, stained knives, and non-connected films.

4. Whether you use a standard slicing knife or a disposable blade, you can get the ideal slicing effect.

5. Original imported differential feeding system and life-long maintenance-free cross guide rails to ensure sectioning

6. Precision and thickness are uniform.

7, equipped with a removable chip tray, easy to clean.

How the slicer works

The working principle of the slicer is relatively simple. By using the sharp cutting surface of the slicer, the object and material are cut into pieces according to the proportion or width of the point. To be suitable for production or pharmaceutical or other uses.

Different microtome sections have different methods, such as processing cells or tissues in the experiment, which is convenient for observation experiments with a microscope. Rotary and slide-away microtome for optical microscopes ^[3]

In the paper industry, slicers are also required, which are suitable for knife-type slicers, drum slicers, and spiral slicers. Knife disc slicer is composed of knife disc, casing, feeding trough and transmission device. The working principle is to use a heavy knife disc to act as a flywheel to stabilize the slice.

Another is to cut the polymer strip into particles. This requires a special slicer, which is composed of a guide plate, a feeding roller, a pressure roller, and a rotary cutter head. The working principle is: the cutter head is driven by a continuously variable transmission, the feeding roller is driven by the cutter head through a set of conversion gears, and the cutter head is equipped with multiple blades according to the size of the pellet. The conversion gear can be changed by itself to change the cut-off length and the continuously variable transmission can change the unique speed of the belt.

Microtome type

There are five basic types of slicers, which can be divided according to their structure:

(1) Shaker type slicer

(2) Rotary slicer

(3) Sliding slicer

(4) Push type (sled type) slicer

(5) Frozen microtome.

The most commonly used is a rotary microtome.

The main features of the slicer

l Compact and independent control panel effectively controls all important operations.

l The built-in control panel and the independent control panel are fully synchronized. The LCD display can indicate the total slice thickness and total slice thickness, trimming thickness, slice thickness, and prompt important operating states.

l Two sample vertical stop positions (up, down)

l Five slice modes: single, continuous, step, half knife, programming

l The slice speed is automatically adjusted according to the slice thickness.

l The trimming function can be turned off. In the automatic state, the parameters of the trimming block are automatically adjusted. In the manual state, the parameters of the trimming block can be determined by programming.

l The slice thickness and trimming thickness can be selected and stored independently.

l The visual and audible signals indicate the forward and backward limits and the remaining injection distance during the injection.

Optional cold light source, magnifying glass or stereo microscope to meet the needs of semi-thin sectioning. The optional ultra-low temperature liquid nitrogen freezing system can perform semi-thin sectioning of special samples.

Microtome considerations

1. Use the "Keyboard Lock Button" on the control panel to lock the keyboard

2. When shutting down, the glass window should be opened, and the standby unit should be completely dry before turning on the machine.

3. It is strictly forbidden to turn off the box compressor at will, otherwise it will seriously affect your work

Technical parameters of slicer

1. Diameter of sawing test piece of slicer: 50, 75, 100
2. Length of sawing test piece: more than 40mm
3. Spindle speed: 2300r / min
4. Motor rated power: 2.2KW voltage 380V
5. Saw blade diameter: 400 mm
6. Dimensions: 900 × 460 × 830mm
Cutting test piece length: 47.5-205mm
7. Machine quality: 150Kg

1. Handle switch 2. Diamond saw blade 3. Cooling water pipe 4. Fixture 5. Clamping collar 6. Water inlet nozzle 7. Movable caster 8. Sink 9. Support 10. Three-phase asynchronous motor 11. Contactor 12. protecting mask