What Is Sugar Homeostasis?

Glucose clamp technology is a method for quantitative detection of insulin secretion and insulin resistance. Glucose clamp technology was first discussed by Andres and colleagues in 1966 and is considered to be the latest glucose homeostasis measurement technology today.

Glucose clamp

Hyperinsulin-normal blood glucose clamp

Peripheral venous infusion increased the subject's serum insulin level to approximately 100 muU / ml and maintained it at this level. Subjects were given a 20% glucose infusion and the blood glucose concentration was maintained at a normal level. The hyperinsulin-normal blood glucose clamp method can be used to assess the sensitivity of peripheral tissues to insulin.

High glucose variable clamp

The researchers raised blood glucose levels to 125 mg / dl (equivalent to 7.0 mmol / l) by blood glucose perfusion. By controlling the glucose perfusion, the blood glucose level is maintained at a high level, and the glucose metabolism rate is evaluated by detecting the glucose perfusion amount under the condition that the blood glucose is maintained at a high level. High glucose variable clamp can evaluate the sensitivity of cells to glucose.

Check your blood glucose level every five minutes. Be sure to make enough glucose perfusion to ensure continuous blood glucose levels. In a normal blood glucose state, the glucose perfusion rate is equal to the glucose uptake of all tissues in the body.

In a 30-minute measurement, the glucose perfusion rate is determined by insulin sensitivity. If the perfusion is higher (7.5 mg / min or higher), then it is usually insulin sensitive; the lower perfusion (4.0 mg / min or lower) usually indicates that you are insulin resistant. The 4.0-7.5 mg / min stage has no specific pathological definition, usually implying impaired glucose tolerance and is a marker of early insulin resistance.

1.Andres R, Swerdloff R, Pozefsky T, Coleman D. Manual feedback technique for control of glucose concentration. In: Skeggs LT Jr, ed. Automation in analytic chemistry. New York: Medaid, Inc .; 1966, 486501.

2. Linda von Wartburg, What's a Glucose Clamp, Anyway? Diabetes Health. Nov 7, 2007.