What are the steps in the Elisa procedure?

The procedure of Elisa begins at the clinic, with a blood sample being taken from the patient. This is followed by a few steps using the ELISA test kit in the laboratory. The test detects the presence of antibodies or antigens for diseases such as HIV, in the blood. The steps include adding different substances and washing, and the results are generally measured by changing color.

The enzyme -connected immunosorbent test (ELISA), which is also referred to as the EIGA enzyme (EIA), is used as a diagnostic test. It is best known as a diagnostic test for HIV. The test plate usually consists of 96 small holes into which a specific antigen is immobilized. If the antibody is present in the serum sample, it binds to antigen, allowing detection to be detected through different steps of ELISA.

The first step in the Elisa procedure is to get a patient sample. This is done by a nurse under sterile conditions. Blood is usually taken from the arm arm or hands. A turnstile is placed on the arm to turn the vein and facilitate the procedure and the skincleans where the needle is inserted. When the needle inside, a small prick may be felt and the blood will be taken in the sterile tube.

After submitting the blood sample, it is transferred to the laboratory, where the rest of the ELISA procedure will be carried out by laboratory technician. The blood sample is first added to the ELISA boards. If a specific antibody is present, it binds to antigen. The plate is then washed using a buffer to remove unrestrained antibodies.

Then the second antibody is added, usually the origin of animals that has an enzyme that is attached to it, which binds to the antibody-antiben complex. The board is washed again to remove the excess. The enzyme is color reactive when the substrate is added at the end of the Elisa procedure. It is the measurement of the degree of color change that gives the result.

Elisa procedure should be carefully followed to prevent false results. Poziti is included in most Elisa boardsIncorporation and negative control to minimize these fake results. When testing on HIV, a positive result will usually be followed by a second, confirming test, often of a different type, such as Western Blot.